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Chlorophyll Fluorescence >
Continuous Excitation
> Pocket PEA Introduction
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Introduction
The Pocket PEA chlorophyll fluorimeter is suitable for teaching, research and a
wide variety of commercial applications. The robust yet compact hand-held design
provides ease of use and reliable operation.
Samples are conveniently dark adapted prior to measurement using the leafclips
supplied. Easy single key operation fully automates the complete measurement
process from data capture through to calculation and display of the key Fv/Fm
and Performance Index (PI) chlorophyll fluorescence parameters. The rapid 1
second measurement capability and 200 measurement memory capacity make the
Pocket PEA chlorophyll fluorimeter an invaluable tool in large plant screening
programs.
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The chlorophyll fluorescence signal received by the sensor during recording is
digitised in the control unit using a fast 16 bit Analogue/Digital converter
ensuring excellent precision and repeatability of results. The fluorescence
signal is digitised at different rates dependent upon the different phases of
the induction kinetic. Initially, data is sampled at 10µs intervals for the
first 300µseconds. This provides excellent time resolution of Fo and the initial
rise kinetics. The time resolution of digitisation is then switched to slower
acquisition rates as the kinetics of the chlorophyll fluorescence signal slow.
This process provides excellent time resolution of the overall measurement
whilst minimising the size of the data set and thus maximising memory capacity.
Bluetooth wireless transfer conveniently allows records to be transferred in the
field from the Pocket PEA chlorophyll fluorimeter to a suitable PDA/IPAQ or PC
for detailed review and analysis using our custom Windows® Mobile and Windows PC
software.
The Pocket PEA optical interface is mounted directly on to the front of the
Pocket PEA control unit. It consists of a single high intensity focused LED
which is positioned vertically above the sample and provides up to 3500 µmols m-2
s-1 intensity with a peak wavelength of 627nm at the sample surface.
The light emitted from the LED is filtered using an NIR filter to block any
infra red content which could be seen by the detector (known as optical
breakthrough). An optical feedback circuit monitors and corrects changes in the
output intensity of the LED which are caused by internal heat build up in the
LED itself. The circuit also compensates for intensity changes caused by
variation in ambient temperature.
The detector is a highly sensitive PIN photodiode and associated amplifier
circuit. The optical design and filtering ensure that it responds maximally to
the longer wavelength fluorescence signal and blocks the reflected shorter
wavelength LED light used as the source of illumination. The entire optical
assembly is sealed behind a clear glass window which creates a barrier against
moisture and dirt which are inherent problems for field based instruments.
The latest Lithium Polymer battery technology ensures a full day of field usage
and the convenience of rapid (<4hrs) recharge to full capacity using either the
mains charger provided or an optional 12v DC vehicle charger.
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