Oxygraph+ Control Unit

Oxygraph+ electrode control unit

  • PC operated oxygen electrode control unit with USB connectivity
  • Suitable for liquid & gas-phase samples with 0% – 100% oxygen concentration
  • 24-bit high-resolution measurement of oxygen signals removes the requirement for gain & back-off settings
  • Integral systems for measurement of pH & other ion-selective electrode signals with 16-bit resolution
  • System expansion to 8-channels via purchase of additional components
  • Windows® software for data acquisition, hardware control & data analysis
  • Real time 0V – 4.5V analogue output of oxygen electrode signal.

Oxygraph+ Control Unit Overview

The next generation Oxygraph+ oxygen electrode control unit combines striking aesthetics with enhanced features and functionality offering significant advances in flexibility and performance over previous generations of electrode control units. As a complete system, Oxygraph+ provides a convenient yet powerful tool for measurements of oxygen evolution or uptake across a broad range of liquid-phase samples from chloroplast extractions to mitochondrial suspensions with oxygen concentrations up to 100%.

Oxygraph+ Electrode Control Unit | Hansatech Instruments | Oxygen electrode and chlorophyll fluorescence measurement systems for cellular respiration and photosynthesis research

Oxygraph+ offers unrivalled price vs. performance combining simplicity of operation with an enviable feature set. The outstanding flexibility ensures Oxygraph+ is equally useful in both a teaching and research capacity.

24-bit resolution allows detection of minute changes in oxygen tension without needing to apply instrument gain. This results in beautiful, noise-free traces even when zoomed close in on areas of interest. Oxygraph+ allows real-time graphing of signals from auxiliary inputs and ion-selective electrodes. This allows comprehensive analysis of oxygen activity simultaneously with signals such as pH, TPP+, calcium, potassium and hydrogen ions.

Up to 8 individual Oxygraph+ control units may be linked to a single PC and operated simultaneously from OxyTrace+ software providing a powerful, multi-channel system.

OxyTrace+ Software

OxyTrace+ is a multi-function Windows® program supplied with our range of PC-operated electrode control units for system configuration, calibration, data acquisition and analysis.

In liquid-phase systems such as Oxygraph+, Oxytherm+, Chlorolab 2+ and Chlorolab 3+, an automated 2-step calibration routine guides the user quickly and effectively through the system calibration process using electrode values measured from air-saturated and deoxygenated water. For gas-phase systems such as Leaflab 2+, an automated 3-step calibration routine using electrode values measured from ambient air, ambient +/- 1ml of injected/removed ambient air and a further measurement of ambient air is employed.

For electrode control units that provide automated light source control in systems such as Oxytherm+P, Chlorolab 2+, Chlorolab 3+ and Leaflab 2+, OxyTrace+ allows simple configuration of comprehensive PFD tables consisting of up to 20 individual light steps. Light intensity adjustments are performed automatically during the measurement. OxyTrace+ also allows calibration of the light source from a simple software routine. This requires the QTP1+ PAR/temperature sensor to be connected to the rear of the electrode control unit and placed into the reaction vessel prior to the addition of any liquids. In the Leaflab 2+ gas-phase system, calibration of the LH36/2R light source requires manual input of measured values at each individual calibration intensity step using the QSRED quantum sensor.

OxyTrace+ Software | Hansatech Instruments | Oxygen electrode and chlorophyll fluorescence measurement systems for cellular respiration and photosynthesis research

A tabbed interface allows a simple transition between the different data views including oxygen electrode (and if configured, auxiliary and external ion-selective electrode) realtime output, a split screen showing realtime rate of change above the oxygen signal and tabulated numerical data.

Post-acquisition analysis tools allow automatic calculation of oxygen rates from user-defined rate intervals. For Oxytherm+P, Chlorolab 2+, Chlorolab 3+ and Leaflab 2+ systems, additional analysis tools automatically calculate rates of change for defined PFD light steps with a calculation of quantum yield presented at the end of a measurement. All files are saved as Comma Separated Values (CSV) data files opening effortlessly in external data processing packages such as MS Excel®.

OxyTrace+ will run on all supported Microsoft operating systems.

Technical Specifications

  • Measuring range:
    • Oxygen: 0% – 100%
    • pH: 0pH – 14pH
    • Aux: 0V – 4.096V
  • Signal inputs:
    • S1 O2 electrode (SMB)
    • pH/ISE (BNC)
    • Auxiliary (8-pin Mini-Din)
  • Resolution:
    • Oxygen: 0.0003% (24-bit)
    • pH: 0.0006pH (16-bit)
    • Aux: 62.5µV/bit (16-bit)
  • Polarising voltage: 700mV
  • Input sensitivity: 0nA – 9000nA
  • Magnetic stirrer: Software controlled 150rpm – 900rpm in % steps
  • Sampling rate: 0.1 – 10 readings/s
  • Electronics:
    • Microcontroller: 16-bit high-performance CPU running at 32 MHz
    • ADC: Dual, Low-power, 16/24-bit Sigma Delta
  • Communications: USB2.0
  • Analogue output: 0V – 4.5V O2 signal
  • Dimensions (HWD): 200mm x 110mm x 60mm
  • Weight: 0.45 Kg
  • Power: 12V dc @ 100mA, 90VAC – 264VAC @ 1A.