Oxyview 1 System

Liquid-phase oxygen electrode teaching system

  • Convenient, low cost system for teaching of photosynthesis research & cellular respiration measurements using the oxygen electrode
  • Clear cast acrylic DW1/AD oxygen electrode chamber with integral Clark-type polarographic oxygen electrode
  • Oxyview electrode control unit with integral magnetic stirrer
  • Oxygen content of sample displayed as digital or percentage value
  • User-friendly configuration via a responsive 4-button control panel & a series of comprehensive menu screens
  • Analogue output for connection to a chart recorder or other external recording device
  • Minimal footprint (90mm x 135mm) allowing multiple units where bench space is limited

The Oxyview 1 liquid-phase oxygen electrode teaching system has been designed in response to the ever-increasing demand for a low-cost system for teaching photosynthesis and respiration measurements using oxygen electrode techniques. The system is designed to be used in a busy teaching environment but is equally at home in a modern research facility.

The Oxyview 1 system consists of a highly sensitive S1 Clark-type polarographic oxygen electrode disc mounted within a DW1/AD electrode chamber and connected to the new Oxyview electrode control unit. The DW1/AD provides a highly versatile solution to measurements of dissolved oxygen in liquid-phase samples of between 0.2 – 2.5ml with its clear cast acrylic construction providing excellent sample visibility and uniform illumination. Precise temperature control of the sample and electrode disc can be achieved by connecting the water jacket of the DW1/AD to a thermoregulated circulating water bath.

The individual components of the Oxyview 1 System are described below:

S1 Oxygen Electrode Disc

Since its original design in the early 1970s by Tom Delieu and David Walker, the S1 Clark-type oxygen electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc and is prepared for use by trapping a layer of 50% saturated KCl solution beneath an oxygen-permeable PTFE membrane. A paper spacer placed beneath the membrane acts as a wick to provide a uniform layer of electrolyte between anode and cathode.

S1 Oxygen Electrode Disc | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

When a small voltage is applied across these electrodes (with the platinum negative with respect to the silver), the current which flows is at first negligible and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H2O2 so that the polarity tends to discharge as electrons are donated to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to the oxygen consumed at the cathode.

S1 Oxygen Electrode Disc Reactions | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration.

DW1/AD Electrode Chamber

A component that has proved itself time and again over 4 decades, the DW1/AD electrode chamber offers quality and versatility in measurements of dissolved oxygen. Developed in conjunction with the great Prof. David Walker, the DW1/AD provides a highly flexible solution for a wide range of applications covering both teaching and research in plant and biomedical science.

DW1/AD Electrode Chamber | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

The reaction vessel of the DW1/AD is constructed from precision-bore borosilicate glass tube with a prepared S1 electrode disc forming the floor of the reaction vessel. Precision temperature control of sample and sensor is delivered via a concentric water jacket with suitable connection ports for a thermoregulated circulating water bath.

DW1/AD is fitted with a gas-tight plunger with a stoppered central bore. The height of the plunger may be adjusted easily to suit liquid-phase sample volumes of between 0.2ml – 2.5ml whilst the central bore easily accommodates Hamilton type syringes allowing additions/subtractions to/from the reaction vessel during an experiment.

The clear acrylic construction allows high sample visibility and uniform sample illumination using external light sources where necessary.

System Components

Oxyview 1 systems are supplied with the following components:

  • DW1/AD: Liquid-phase electrode chamber
  • OXYV1: Electrode control unit
  • A2: Membrane applicator to assist with smooth application of electrode membrane
  • A3: Top plate key and alignment jig to dismantle and reassemble DW1/AD for cleaning
  • S2/P: Pack of 5 magnetic followers
  • S3: Pack of 2 replacement borosilicate glass reaction vessels
  • S4: PTFE membrane (0.0125mm x 25mm x 33m)
  • S7A: Replacement O-rings for electrode chamber
  • S16: Cleaning kit for the S1 electrode disc.

Technical Specifications

Oxyview 1 electrode control unit

  • Measuring range: Oxygen: 0% – 100%
  • Signal inputs: Oxygen electrode (SMB)
  • Resolution: 10 x 10-6 μmols/ml at 20°C
  • Polarising voltage: Selectable between 0.4V – 0.9V (0.7V recommended default)
  • Gain: Coarse: x1, x2, x5, x10, x20, x50, x100. Fine: 1mV steps
  • Back off: Signal back off in 1mV steps
  • Analogue output: 0V – 5V electorde signal
  • Dimensions (HWD): 90mm x 135mm x 85mm
  • Weight: 320g
  • Power: 95V – 260V universal input mains supply. Output 12V DC 2.5A

DW1/AD electrode chamber

  • Suitability: Liquid-phase respiration/photosynthesis
  • Construction: Clear cast acrylic
  • Sample chamber: Precision-bore, borosilicate glass tube
  • Sample volume: 0.2ml – 2.5ml
  • Temperature control: Water jacket connected to thermoregulated circulating water bath
  • Dimensions (DH): 65mm x 105mm
  • Weight: 100g
  • Plunger: Gas-tight, variable-height plunger assembly with stoppered central bore for sample additions.

S1 oxygen electrode disc

  • Electrode type: Clark-type polarographic oxygen sensor
  • Electrode output: Typically 1.6µA at 21% O2
  • Residual current: Typically 0.04µA in 0% O2
  • Response time: 10 – 90% typically <5 seconds
  • Oxygen consumption: Typically <0.015µmol/hr-1

Publications

One of the extensions of the Google search facility is Google Scholar. It allows you to search through vast archives of peer-reviewed published papers and journals that have been posted online.

Use the tool below to enter search terms as required. As an example, hansatech instruments Oxyview 1 System has already been entered into the search box. Press the "Search" button to view the Google Scholar results for this search string.

Admittedly, some of the results link to journals which require subscription in order to view the publications but even so, we have found this facility to be a valuable tool.

Google Scholar

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