Chloroview 1 System

Entry-level system for the study of photosynthesis & respiration in liquid-phase samples under illumination

  • Oxygen electrode control and signal acquisition via Oxyview electrode control unit
  • DW1 oxygen electrode unit with integral oxygen electrode
  • Oxygen signal output from Oxyview to recording device via 0V – 5V analogue output
  • Suitable for liquid-phase samples between 0.2ml – 2.5ml with 0% – 100% oxygen concentration
  • Illumination via LS2 high-intensity halogen white light source
  • Quantitherm light/temperature sensor for light source calibration.

Chloroview 1 is an entry level system for studies of photosynthesis and respiration from liquid-phase suspension samples under actinic illumination. The system is ideally suited to teaching environments for under and post-grad plant biology studies of the photosynthetic processes but is equally at home in research facilities where demands on equipment performance are high.

The individual components of the Chloroview 1 System are described below:

S1 Oxygen Electrode Disc

Since its original design in the early 1970s by Tom Delieu and David Walker, the S1 Clark-type oxygen electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc and is prepared for use by trapping a layer of 50% saturated KCl solution beneath an oxygen-permeable PTFE membrane. A paper spacer placed beneath the membrane acts as a wick to provide a uniform layer of electrolyte between anode and cathode.

S1 Oxygen Electrode Disc | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

When a small voltage is applied across these electrodes (with the platinum negative with respect to the silver), the current which flows is at first negligible and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H2O2 so that the polarity tends to discharge as electrons are donated to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to the oxygen consumed at the cathode.

S1 Oxygen Electrode Disc Reactions | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration.

DW1 Electrode Chamber

A component that has proved itself time and again over 4 decades, the DW1 electrode chamber offers quality and versatility in measurements of dissolved oxygen. Developed in conjunction with the great Prof. David Walker, the DW1 provides a highly flexible solution for a wide range of applications covering both teaching and research in plant and biomedical science.

DW1/AD Electrode Chamber | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

The reaction vessel of the DW1 is constructed from precision-bore borosilicate glass tube with a prepared S1 electrode disc forming the floor of the reaction vessel. Precision temperature control of sample and sensor is delivered via a concentric water jacket with suitable connection ports for a thermoregulated circulating water bath.

DW1 is fitted with a plunger with a stoppered central bore. The height of the plunger may be adjusted easily to suit liquid-phase sample volumes of between 0.2ml – 2.5ml whilst the central bore easily accommodates Hamilton-type syringes allowing additions/subtractions to/from the reaction vessel during an experiment.

The clear cast acrylic construction allows high sample visibility and uniform sample illumination using external light sources where necessary.

Oxyview Control Unit

The OXYV1 Oxyview control unit has been designed as a convenient, low-cost oxygen electrode control unit for teaching studies of photosynthesis and cellular respiration using the oxygen electrode measurement technique.

The Oxyview control unit is fully compatible with the range of oxygen electrode chambers produced by Hansatech Instruments thus allowing a wide range of different assays to be performed in both liquid and gas-phases.

Oxyview Control Unit | Hansatech Instruments | Oxygen electrode and chlorophyll fluorescence measurement systems for cellular respiration and photosynthesis research

The Oxyview is configured and controlled via a front mounted control panel featuring 4 touch-sensitive buttons. Configuration is achieved by navigating through a series of simple menu screens and following the displayed guidelines for each step of the setup process. These configuration steps include setting of the stirrer speed (for liquid-phase measurements) and back-off and gain settings. Once configured, the Oxyview control unit provides an accurate and stable reading of the oxygen content of the sample in question.

The Oxyview control unit is powered by a 12V DC wall cube which connects directly to the rear of the unit. Also at the rear is a 0V – 5V analogue output. This allows the measured values from the control unit to be logged to an external recording device such as a chart recorder or similar datalogger accepting a 0V – 5V analogue input.

LS2 White Light Source

LS2 white light source | Hansatech Instruments | Oxygen electrode and chlorophyll fluorescence measurement systems for cellular respiration and photosynthesis research

The LS2 light source is a high-intensity (100W) tungsten-halogen light source which is powered from a stabilised power supply.

The lamp housing contains a cooling fan, infrared reducing “Hot-Mirror” and optics to provide light with minimum variation of intensity across the beam and little divergence from parallel. 2 slots are provided to accept 50mm square optical filters or a manual shutter-plate.

A range of light intensity steps may be achieved by adding permutations of the 4 A5 neutral density filters supplied (0.1,0.3,0.6 and 1.0 O.D.) to attenuate the light intensity. Maximum sample illumination intensities vary depending on the type of electrode chamber in use.

When used with the DW1 electrode chamber the LS2 can achieve a maximum intensity of 1,800 µmol m-2 s-1 when mounted horizontally with respect to the electrode chamber.

Quantitherm PAR/Temp Sensor


The Quantitherm consists of the QRT1 handheld display unit combined with the QTP1+ probe sensor. For use as a calibration tool for the LS2 light source, the QTP1+ probe connects directly to the QRT1 control unit allowing real-time measurement of light intensity via the built-in screen. The light intensity of the LS2 Light source can then be adjusted using the neutral density filters provided.

The QRT1 quantum sensor provides a displayed resolution of 1 µmol m-2 s-1 throughout the 0 µmol m-2 s-1 – 5,000 µmol m-2 s-1 range and up to a maximum of 50,000 µmol m-2 s-1 with a displayed resolution of 10 µmol m-2 s-1.

QTP1 probe

The QTP1+ probe sensor connects to the QRT1 control unit via a Mini-DIN connection. The probe is designed to be mounted directly into the DW1, DW1/AD, DW2/2, DW3 and Oxytherm+ electrode chambers via the use of suitable mounting collars (mounting collar for DW1 and DW2/2 supplied, collars for DW3 and Oxytherm+ supplied with respective items).

Despite being intended for light source calibration in liquid-phase oxygen electrode chambers, the PAR/temperature probe sensor must not be submerged in liquid. Although the probe is splash proof, prolonged contact with liquid will irreversibly damage the sensor. Calibration of light sources should be performed prior to the addition of samples. Damage caused by submersion will not be covered by warranty.

The probe consists of a PAR quantum sensor and a thermistor bead for temperature measurement and is constructed from stainless steel and acetal. Temperature is measured by an RT curve matched-type glass bead thermistor mounted centrally in the probe tip. Photosynthetically Active Radiation (PAR) levels are determined by a quantum sensor located in the side wall of the probe.

The QTP1+ probe may also be connected directly to the rear of the Oxylab+ oxygen electrode control unit. OxyTrace+ software plots the temperature signal from the QTP1+ in real-time as a chart recorder emulation on the same screen as the signal from the S1 oxygen electrode disc. PAR values are also displayed in the OxyTrace+ software light source calibration routine providing a convenient display of measured values during the light source calibration process.

System Components

Chloroview 1 Systems are supplied with the following components:

  • DW1: Oxygen electrode chamber
  • OXYV1: Oxyview electrode control unit with integral magnetic stirrer
  • S1: Oxygen electrode disc and SMB-SMB connection cable
  • LS2: Tungsten-halogen white light source
  • QRT1: Quantitherm PAR/temperature sensor
  • A2: Membrane applicator to assist with smooth application of electrode membrane
  • A3: Top plate key and alignment jig to dismantle and reassemble DW1 for cleaning
  • A5: Set of 5 neutral-density filters
  • S2/P: Pack of 5 magnetic followers
  • S3: Pack of 2 replacement borosilicate glass reaction vessels
  • S4: Reel of PTFE membrane (0.0125mm x 25mm x 33m)
  • S7A: Set of replacement O-rings for DW1
  • S16: Cleaning kit for the S1 electrode disc.

Technical Specifications

Oxyview 1 electrode control unit

  • Measuring range: Oxygen: 0% – 100%
  • Signal inputs: Oxygen electrode (SMB)
  • Resolution: 10 x 10-6 μmols/ml at 20°C
  • Polarising voltage: Selectable between 0.4V – 0.9V (0.7V recommended default)
  • Gain: Coarse: x1, x2, x5, x10, x20, x50, x100. Fine: 1mV steps
  • Back off: Signal back off in 1mV steps
  • Analogue output: 0V – 5V electorde signal
  • Dimensions (HWD): 90mm x 135mm x 85mm
  • Weight: 320g
  • Power: 95V – 260V universal input mains supply. Output 12V DC 2.5A

DW1 electrode chamber

  • Suitability: Liquid-phase respiration/photosynthesis
  • Construction: Clear cast acrylic
  • Sample chamber: Precision-bore, borosilicate glass tube
  • Sample volume: 0.2ml – 2.5ml
  • Temperature control: Water jacket connected to thermoregulated circulating water bath
  • Dimensions (DH): 65mm x 105mm
  • Weight: 100g
  • Plunger: Variable-height plunger assembly with central bore for sample additions.

S1 oxygen electrode disc

  • Electrode type: Clark-type polarographic oxygen sensor
  • Electrode output: Typically 1.6µA at 21% O2
  • Residual current: Typically 0.04µA in 0% O2
  • Response time: 10 – 90% typically <5 seconds
  • Oxygen consumption: Typically <0.015µmol/hr-1

LS2 light source

  • Lamp Type: 100W tungsten-halogen (50W available on request)
  • Power Supply: Mains, stabilised power supply. 12V DC 10A. 120/240V 60/60Hz
  • Intensity Adjustment: Via combinations of 4 neutral-density filters (supplied)
  • Max Intensity in DW1: 1,800 μmol m-2 s-1
  • Dimensions:
    • Light housing: 145mm x 65mm x 75mm. Weight 1.0kg
    • Power supply: 86 mmx 150mm x 140mm. Weight 1.4kg.

QRT1 PAR/temperature sensor

  • Measuring range: 0 μmol m-2 s-1 – 50,000 μmol m-2 s-1 (+/- 5%) in 2 ranges (0 μmol m-2 s-1 – 5,000 μmol m-2 s-1 & 0 – 50,000 μmol m-2 s-1) in 400nm – 700nm band
  • Resolution:
    • 1 µmol m-2 s-1 at 0 µmol m-2 s-1 – 5,000 µmol m-2 s-1
    • 10 μmol m-2 s-1 at 5,001 µmol m-2 s-1 – 50,000 µmol m-2 s-1
  • PAR sensor: Silicon photodiode and optical filter with white acetal diffuser
  • Temperature sensor: RT curve-matched bead thermistor. 0°C – 50°C/32°F – 122°F (0.02°C resolution)
  • Signal display: Hand-held display unit. 16 x 2 LCD display. 0V – 5V analogue output of PAR/temperature values
  • Power requirements: 4 x 1.5V AA (LR6) cells. Typically 100 hours battery life
  • Dimensions:
    • Display: 146mm x 92mm x 32mm. Weight 300g
    • QTP1+: 9.5mm x 107mm. Weight 50g

Publications

One of the extensions of the Google search facility is Google Scholar. It allows you to search through vast archives of peer-reviewed published papers and journals that have been posted online.

Use the tool below to enter search terms as required. As an example, hansatech instruments Chloroview 1 System has already been entered into the search box. Press the "Search" button to view the Google Scholar results for this search string.

Admittedly, some of the results link to journals which require subscription in order to view the publications but even so, we have found this facility to be a valuable tool.

Google Scholar

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