Oxygraph+ System

Liquid-phase photosynthesis & respiration measurement

  • PC operated oxygen electrode control unit with USB connectivity
  • Clear cast acrylic DW1/AD oxygen electrode chamber with integral Clark-type polarographic oxygen electrode
  • Suitable for liquid-phase samples with 0% – 100% oxygen concentration
  • 24-bit high-resolution measurement of oxygen signals
  • Integral systems for measurement of pH & other ion-selective electrode signals with 16-bit resolution
  • System expansion to 8-channels via purchase of additional Oxygraph+ systems
  • OxyTrace+ Windows® software for data acquisition, hardware control & data analysis
  • Real time 0V – 4.5V analogue output of oxygen electrode signal

The Oxygraph+ oxygen electrode system provides PC control of oxygen uptake or evolution measurements across a broad range of applications from studies of mitochondria and cellular respiration to measurements of isolated chloroplast suspensions in photosynthesis research applications with up to 100% oxygen concentration.

The Oxygraph+ system consists of a highly sensitive S1 Clark-type polarographic oxygen electrode disc mounted within a DW1/AD electrode chamber and connected to the new Oxygraph+ electrode control unit. The DW1/AD provides a highly versatile solution to measurements of dissolved oxygen in liquid-phase samples of between 0.2ml – 2.5ml with its clear cast acrylic construction providing excellent sample visibility & uniform illumination. Precise temperature control of the sample and electrode disc can be achieved by connecting the water jacket of the DW1/AD to a thermoregulated circulating water bath.

The individual components of the Oxygraph+ System are described below.

S1 Oxygen Electrode Disc

Since its original design in the early 1970s by Tom Delieu and David Walker, the S1 Clark-type oxygen electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc and is prepared for use by trapping a layer of 50% saturated KCl solution beneath an oxygen-permeable PTFE membrane. A paper spacer placed beneath the membrane acts as a wick to provide a uniform layer of electrolyte between anode and cathode.

S1 Oxygen Electrode Disc | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

When a small voltage is applied across these electrodes (with the platinum negative with respect to the silver), the current which flows is at first negligible and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H2O2 so that the polarity tends to discharge as electrons are donated to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to the oxygen consumed at the cathode.

S1 Oxygen Electrode Disc Reactions | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration.

DW1/AD Electrode Chamber

A component that has proved itself time and again over 4 decades, the DW1/AD electrode chamber offers quality and versatility in measurements of dissolved oxygen. Developed in conjunction with the great Prof. David Walker, the DW1/AD provides a highly flexible solution for a wide range of applications covering both teaching and research in plant and biomedical science.

DW1/AD Electrode Chamber | Oxygen electrode & chlorophyll fluorescence measurement systems for cellular respiration & photosynthesis research

The reaction vessel of the DW1/AD is constructed from precision-bore borosilicate glass tube with a prepared S1 electrode disc forming the floor of the reaction vessel. Precision temperature control of sample and sensor is delivered via a concentric water jacket with suitable connection ports for a thermoregulated circulating water bath.

DW1/AD is fitted with a gas-tight plunger with a stoppered central bore. The height of the plunger may be adjusted easily to suit liquid-phase sample volumes of between 0.2ml – 2.5ml whilst the central bore easily accommodates Hamilton type syringes allowing additions/subtractions to/from the reaction vessel during an experiment.

The clear acrylic construction allows high sample visibility and uniform sample illumination using external light sources where necessary.

Oxygraph+ Control Unit

The next generation Oxygraph+ oxygen electrode control unit combines striking aesthetics with enhanced features and functionality offering significant advances in flexibility and performance over previous generations of electrode control units. As a complete system, Oxygraph+ provides a convenient yet powerful tool for measurements of oxygen evolution or uptake across a broad range of liquid-phase samples from chloroplast extractions to mitochondrial suspensions with oxygen concentrations up to 100%.

Oxygraph+ Electrode Control Unit | Hansatech Instruments | Oxygen electrode and chlorophyll fluorescence measurement systems for cellular respiration and photosynthesis research

Oxygraph+ offers unrivalled price vs. performance combining simplicity of operation with an enviable feature set. The outstanding flexibility ensures Oxygraph+ is equally useful in both a teaching and research capacity.

24-bit resolution allows detection of minute changes in oxygen tension without needing to apply instrument gain. This results in beautiful, noise-free traces even when zoomed close in on areas of interest. Oxygraph+ allows real-time graphing of signals from auxiliary inputs and ion-selective electrodes. This allows comprehensive analysis of oxygen activity simultaneously with signals such as pH, TPP+, calcium, potassium and hydrogen ions.

Up to 8 individual Oxygraph+ control units may be linked to a single PC and operated simultaneously from OxyTrace+ software providing a powerful, multi-channel system.

OxyTrace+ Software

OxyTrace+ is a multi-function Windows® program supplied with our range of PC-operated electrode control units for system configuration, calibration, data acquisition and analysis.

In liquid-phase systems such as Oxygraph+, Oxytherm+, Chlorolab 2+ and Chlorolab 3+, an automated 2-step calibration routine guides the user quickly and effectively through the system calibration process using electrode values measured from air-saturated and deoxygenated water. For gas-phase systems such as Leaflab 2+, an automated 3-step calibration routine using electrode values measured from ambient air, ambient +/- 1ml of injected/removed ambient air and a further measurement of ambient air is employed.

For electrode control units that provide automated light source control in systems such as Oxytherm+P, Chlorolab 2+, Chlorolab 3+ and Leaflab 2+, OxyTrace+ allows simple configuration of comprehensive PFD tables consisting of up to 20 individual light steps. Light intensity adjustments are performed automatically during the measurement. OxyTrace+ also allows calibration of the light source from a simple software routine. This requires the QTP1+ PAR/temperature sensor to be connected to the rear of the electrode control unit and placed into the reaction vessel prior to the addition of any liquids. In the Leaflab 2+ gas-phase system, calibration of the LH36/2R light source requires manual input of measured values at each individual calibration intensity step using the QSRED quantum sensor.

OxyTrace+ Software | Hansatech Instruments | Oxygen electrode and chlorophyll fluorescence measurement systems for cellular respiration and photosynthesis research

A tabbed interface allows a simple transition between the different data views including oxygen electrode (and if configured, auxiliary and external ion-selective electrode) realtime output, a split screen showing realtime rate of change above the oxygen signal and tabulated numerical data.

Post-acquisition analysis tools allow automatic calculation of oxygen rates from user-defined rate intervals. For Oxytherm+P, Chlorolab 2+, Chlorolab 3+ and Leaflab 2+ systems, additional analysis tools automatically calculate rates of change for defined PFD light steps with a calculation of quantum yield presented at the end of a measurement. All files are saved as Comma Separated Values (CSV) data files opening effortlessly in external data processing packages such as MS Excel®.

OxyTrace+ will run on all supported Microsoft operating systems.

System Components

Oxygraph+ systems are supplied with the following components:

  • DW1/AD: Liquid-phase electrode chamber
  • S1: Oxygen electrode disc and SMB-SMB connection cable
  • OXYG1+: Electrode control unit
  • A2: Membrane applicator to assist with smooth application of electrode membrane
  • A3: Top plate key and alignment jig to dismantle and reassemble DW1/AD for cleaning
  • S2/P: Pack of 5 magnetic followers
  • S3: Pack of 2 replacement borosilicate glass reaction vessels
  • S4: PTFE membrane (0.0125mm x 25mm x 33m)
  • S7A: Replacement O-rings for electrode chamber
  • S16: Cleaning kit for the S1 electrode disc.

Technical Specifications

Oxygraph+ electrode control unit

  • Measuring range:
    • Oxygen: 0% – 100%
    • pH: 0pH – 14pH
    • Aux: 0V – 4.096V
  • Signal inputs:
    • S1 O2 electrode (SMB)
    • pH/ISE (BNC)
    • Auxiliary (8-pin Mini-Din)
  • Resolution:
    • Oxygen: 0.0003% (24-bit)
    • pH: 0.0006pH (16-bit)
    • Aux: 62.5µV/bit (16-bit)
  • Polarising voltage: 700mV
  • Input sensitivity: 0nA – 9000nA
  • Magnetic stirrer: Software controlled 150rpm – 900rpm in % steps
  • Sampling rate: 0.1 – 10 readings/s
  • Electronics:
    • Microcontroller: 16-bit high-performance CPU running at 32 MHz
    • ADC: Dual, Low-power, 16/24-bit Sigma Delta
  • Communications: USB2.0
  • Analogue output: 0V – 4.5V O2 signal
  • Dimensions (HWD): 200mm x 110mm x 60mm
  • Weight: 0.45 Kg
  • Power: 12V dc @ 100mA, 90VAC – 264VAC @ 1A.

DW1/AD electrode chamber

  • Suitability: Liquid-phase respiration/photosynthesis
  • Construction: Clear cast acrylic
  • Sample chamber: Precision-bore, borosilicate glass tube
  • Sample volume: 0.2ml – 2.5ml
  • Temperature control: Water jacket connected to thermoregulated circulating water bath
  • Dimensions (DH): 65mm x 105mm
  • Weight: 100g
  • Plunger: Gas-tight, variable-height plunger assembly with stoppered central bore for sample additions.

S1 oxygen electrode disc

  • Electrode type: Clark-type polarographic oxygen sensor
  • Electrode output: Typically 1.6µA at 21% O2
  • Residual current: Typically 0.04µA in 0% O2
  • Response time: 10 – 90% typically <5 seconds
  • Oxygen consumption: Typically <0.015µmol/hr-1


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